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KMID : 0368419890320030145
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Journal of Plant Biology
1989 Volume.32 No. 3 p.145 ~ p.150
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Plant Regeneration through Somatic Embryogenesis from Mature Zygotic Embryos of Ginseng(Punax ginseng C. A. Meyer) and Flowering of Plantlets
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李幸順/Lee, Haeng Soon
李光雄/梁承均/田在興/劉長烈/Lee, Kwang Woong/Yang, Seung Gyun/Jeon, Jae Heung/Liu, Jang Ryol
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Abstract
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Mature zygotic embryos dissected from ginseng(Panax ginseng C.A. Meyer) seeds were cultured on Murashige and Skoog´s (MS) medium containing various concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and kinetin. Somatic embryos were induced directly from cotyledonary tissue or from intervening callus. The induction frequency of somatic embryos was up to 55%. Upon transfer to half-strength MS medium supplemented with 1 ㎎/l 6-benzyladenine(BA) and 1 ㎎/l GA_3, most somatic embryos dveloped into plantlets. Over 50% of the plantlets flowered after 4 weeks of culture and then a few bore immature fruits in vitro. Therefore, it is suggested that the juvenility of the ginseng tissue which give rise to somatic embryos does not interfere with in vitro flowering of their regenerated plantlets.
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